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Science

Ten minutes,
not three days.

Speed and simplicity, not a new target. The test measures progesterone — the same analyte as a lab ELISA — in a couple of drops of camel whole blood. No separation, no filtration, no lab. Pregnant camels exceed 3 ng/mL by day 20 post mating; the 2 ng/mL cutoff turns the strip positive.

Progesterone lateral-flow assay diagram
The biology

Progesterone, measured in the field.

In a pregnant camel, the corpus luteum sustains progesterone production well past the non-pregnant cycle drop. By day 20 post mating, progesterone exceeds 3 ng/mL. The test uses a 2 ng/mL cutoff — a conservative floor that preserves sensitivity while rejecting baseline noise.

Progesterone target

Competitive lateral-flow immunoassay with an anti-progesterone monoclonal antibody on the test line.

2 ng/mL cutoff

The test line fades above 2 ng/mL. Pregnant camels at day 20 are typically above 3 ng/mL — a clean margin.

Whole blood only

A couple of drops of whole blood from the jugular. No centrifuge, no separation, no filtration — the strip handles sample preparation in the membrane.

Detection timeline

When the test turns positive.

Progesterone stays elevated through gestation in pregnant camels and drops back to baseline within two weeks in non-pregnant cycles.

Day0

Mating

Reference day. Progesterone baseline, < 1 ng/mL.

Day14

Corpus luteum phase

Progesterone rising in both pregnant and non-pregnant camels — not yet discriminating.

Day20

Pregnant: > 3 ng/mL · Cutoff: 2 ng/mL

In pregnant camels, progesterone exceeds 3 ng/mL. In non-pregnant cycles, it has already dropped below the 2 ng/mL cutoff. The test reads positive.

Detection window opens
Day35

Ultrasound becomes reliable

Transrectal ultrasound by a trained veterinarian can confirm a vesicle.

Day60

Conventional confirmation

The earliest point at which most existing field methods can confirm pregnancy.

Comparison

The same day as ELISA. Without the lab.

Progesterone ELISA is the established reference method at day 20 — but it requires a laboratory, trained technicians, and one to three days to return a result. The test delivers the same answer in the same field visit.

The test
ELISA (lab)
Ultrasound
Earliest detection
20 days
20 days
35 days
Time to result
10 minutes
1–3 days
Real-time, expert-dependent
Sample
Whole blood, couple of drops
Serum (requires centrifugation)
N/A
Field deployable
Yes
Lab only
Equipment + training
Operator skill
Herder, no training
Lab technician
Veterinarian
Cost per test
$25
$25 + shipping + lab time
$80–$200 vet visit
Validation

Will be validated on >500 samples in the UAE.

>500
Planned field samples, UAE
Day 20
Detection window post mating
ELISA and Ultrasound
Reference methods for validation
Publications

Peer-reviewed data.

Validation results and assay performance data will be published in peer-reviewed journals following completion of the UAE field study.

Coming soon

Field validation manuscript in preparation.